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Cell-based and molecular assays At the molecular level, receptor pharmacology is quantified with functional assays such as cyclic-AMP accumulation readouts (often using time-resolved Frster resonance energy transfer or luciferase reporter systems), radioligand competition binding to measure affinity, and -arrestin recruitment assays to assess receptor internalization and signaling bias

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Additionally, the co-localization of SLC7A11 and Sec24 C was verified by immunofluorescence assays (Fig

This assessment was carried out using the Dummett and Gupta Oral Pigmentation Indexs predetermined score criteria [] [Table 1]

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