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The knockdown efficiency at 48 h or 72 h post-transfection was examined using reverse transcription-quantitative PCR (RT-qPCR) and western blot analyses

For DBS and VAMS just a simple extraction step with organic solvent mixtures is needed prior to HPLC-MS/MS analysis but, depending on the blood volume sampled, lower analytical performance has to be taken into account (Osteresch et al

PLOS ONE, 15(9), e0239252

[DOI] [PubMed] [Google Scholar] 66.Ribas V., Garca-Ruiz C., Fernndez-Checa J.C

Lee et al., 2007

Reconstitution math for 100mg Standard reconstitution adds 3mL of bacteriostatic water to 100mg, creating a concentration of approximately 33.3mg/mL