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Using carboxyfluorescein (CF) as a fluorescence tracer, Rip et al

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Nrf2 knockdown prevents NFT-dependent increase in cellular glutathione levels To examine the correlation between Nrf2 activation and the increase in glutathione levels after NFT treatment, Nrf2 protein levels were experimentally lowered by siRNA-mediated knockdown, which was confirmed by Western blotting (Fig

Briefly, cells were initially seeded onto Geltrex-coated tissue culture plates as single cells after incubation with Accutase (Life Technologies)