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Maintain proper storage during transport

Apparently, one of the reasons for this change was to align the Japanese cards with the international ones

Mixing two distinct peptide powders into a single vial can cause them to bind to each other, altering their chemical structures and rendering them ineffective

Protein digestion Protein concentration was estimated by BCA assay (Pierce) and the lysates were digested with Lys-C (Wako) in an enzyme/protein ratio of 1:100 (w/w) for 1 h followed by a threefold dilution with 25 mM Tris, pH 8.5 to 2 M GdmCl and further digested overnight with trypsin (Sigma-Aldrich) 1:100 (w:w)

Topical use is the most common