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Samples were frozen and dried in a Speed Vac (Labconco), then re-suspended in 0.1% TFA before MS analysis

Gingivalis Exacerbates Autoimmune Encephalomyelitis

doi: 10.1073/pnas.1812821115

Subjects and methods The subjects were 30 healthy adult women aged 30 to 50 years

Sterilization: Wipe the rubber stoppers of both the Tirzepatide vial and the Bac water vial with alcohol wipes and allow them to air dry completely to maintain a sterile research environment

Proteins in other models did not display clear biased distribution of phases, likely because of the relatively low number of rhythmic proteins compared with mRNAs (Figures 1H and Supplemental Figure 1D)