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NaufahuJElliottBMarkivADunning-ForemanPMcGradyMHowardDet al

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Possible Cause: Inhibitors were present in medium Suggested Solution: Do not use antibiotics, EDTA, citrate, phosphate, RPMI, chondroitin sulfate, hyaluronic acid, dextran sulfate, or other sulfated proteoglycans in the growth medium or in the medium used to prepare DNA:transfection reagent complexes

Heterologous expression and sample preparation Recombinant C-terminally LEH 6 -tagged wild-type and mutant roGFP2, N-terminally MRGSH 6 GS-tagged PfGrx variants, and N-terminally MAH 6 VGT-tagged roGFP2 WT -PfGrx WT , roGFP2 WT -PfGrx C88S , and roGFP2 WT -PfGrx C32S/C88S were produced in E