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10.1371/journal.pone.0028378 39 HalliwellB
Solvent mixtures containing methanol, acetonitrile and/or chloroform, with or without water, are commonly used for metabolite extraction in the in vitro toxicometabolomic studies reviewed

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PCR was performed in triplicate with the following thermocycler profile: Denaturation at 95C for 5 min