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Culture medium was replaced by adipogenic medium I containing DMEM/F12 medium (Gibco) supplemented with 33 M biotin (SA), 17 M pantothenate (SA), transferrin (10 g/ml), 20 nM insulin, 100 nM cortisol, 0.2 nM triiodothyronine (T3), 25 nM dexamethasone, 250 M IBMX, and 2 M rosiglitazone

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While at diagnosis of the disease approximately 6% of the patients are found to be Pgp-positive, after therapy (especially treatment using the VAD scheme--vincristine, doxorubicin, dexamethasone) up to 85% resistant to treatment patients become Pgp-expressing [34]

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