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To create the ges-1p::cpt-4 , we ligated a 2 kb ges-1 promoter to the coding region of cpt-4 (cloned from N2 genomic DNA) and unc-54 3UTR using Gibson Assembly

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Oct 2016;13:166-172

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To further investigate, whether NHR1 is a modulator of proteostasis, or just a modulator of polyQ aggregation, we introduced both vlt16 and vlt10 alleles into an synuclein::YFPexpressing strain of worms

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