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Inter-animal variability, calculated as the coefficient of variation (%CV) based on the summed intensities of all detected ions across analysed regions, was 5.28% in negative ion mode and 5.57% in positive ion mode

Overlapping symptoms of IC/BPS and OAB often make the two conditions difficult to distinguish and may complicate subsequent diagnosis and treatment algorithms 4,5

Equilibration was as follows: solvent was decreased from 100% B to 10% B over 0.1 min and held for an additional 0.9 min

Then, take a portion of the ground tissue and add cold protease inhibitors and lysate

The reconstitution process itself is straightforward, but accuracy matters

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