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Targeted and non-targeted metabolomics analysis All sample preparation and instrumental analyses for targeted and non-targeted metabolomic analyses were conducted following the procedures outlined in our previous study 13 , with the exception of the analysis of tricarboxylic acid (TCA) metabolites, which were investigated using our validated in-house method
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After 4 weeks, dose increases occur every 24 weeks based on your tolerability and weight loss response, not on a rigid calendar
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