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The cells were fixed with 4% PFA and 0.2% glutaraldehyde in a buffer containing 30 mM HEPES (pH 7.4), 100 mM NaCl, and 2 mM CaCl 2 for 10 min at room temperature

When staining was performed with anti-GCLC, anti-cleaved caspase3, anti-TSPO (PBR), anti-PSD95 or anti-GSDME antibodies, we applied a fluorescence-indirect tyramide signal amplification (TSA) technique (TSA System

Then this interleukin binds CD8+ NKG2D+ T cells to further stimulate IFN- production through JAK1 and JAK3 pathways, forming a positive feedback loop (Figure 1) that destroys anagen hair follicles and leads to alopecia areata (34)

Nakamura, H., H

Fritsch M, Orfanos CE, Zouboulis CC

In 1990, Frosst et al (28) identified a C677T polymorphism in the MTHFR gene that reduced enzyme activity and altered folate concentrations