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Nonetheless, in clinical practice, the lipid profile is conventionally measured in blood plasma or serum obtained after fasting for at least 8 h, and therefore may not reflect the daily average plasma lipid and lipoprotein concentrations and associated risk of cardiovascular disease

Diabetes 64: 21942206, 2015

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After centrifugation for 15 min at 12,000 g , a 120 l supernatant was mixed with 100 l each of solution 1 (containing 100 mM phenol and 50 mg l 1 sodium nitroprusside) and solution 2 (comprising 0.38 M dibasic sodium phosphate, 125 mM NaOH, and 1% sodium hypochlorite with available chlorine ranging from 10 to 15%)