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As a result, the K271/K290 sites of FoxO3a exhibit high acetylation levels, leading to decreased expression of BIM (a BH3-only protein with the characteristic BH3 domain of the Bcl-2 family that binds to anti-apoptotic proteins, inhibits their function, and thereby promotes the activation of pro-apoptotic proteins such as Bax and Bak), which in turn facilitates the occurrence of apoptosis [198] (Fig

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However, some peptides are poorly soluble at neutral pH and require a mildly acidic solvent such as 0.6% acetic acid water