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Affinities were determined aerobically using in-line probing which monitors ligand induced structural changes in the RNA molecule, therefore values might represent a combination of affinity and allostery

(PubMed) Bai Y, Wang X, Zhao S, Ma C, Cui J, Zheng Y

studied specific proteins at different concentrations in the follicular fluid of patients with advanced endometriosis and found that the transferrin concentration of the three analogues of cDNA FLJ53691, cDNA FLJ54111, and TRF variant Fragment in the follicular fluid decreased

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These changes resulted in elevated intracellular Fe 2+ levels and the downregulation of p-STAT3, SLC7A11, and GPX4 levels (57)