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Histidine and lysine produced significantly more pigment than arginine under identical conditions, and a Box-protective lysine (Boc-Lys), which preserves the -NH 2 group, yielded deeper pigmentation than unprotected lysine, implicating the -amino group of lysine as a key reactive site

In a small proportion of cases, the melanin may be prominent enough to be visualized as foci of dark-brown or black discoloration ( Fig

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