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Front Pharmacol 13:986963

incidence is higher in certain regions where uncontrolled hydroquinone use was prevalent

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Cell pellets were resuspended in the lysis buffer (50 mM Tris pH 7.6, 150 mM NaCl, 5 mM MgCl2, 0.5 mM EDTA, 0.5% NP-40, 10% v/v glycerol with freshly added protease inhibitors) and incubated on ice for 30 min with vortexing every 10 min

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