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Animals Sprague-Dawley rats aged 28 days weighing in the range of 70 to 90 g were purchased from The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

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All data were analyzed by one-way or two-way analysis of variance followed by Student's modified t test with Bonferroni corrections for multiple comparisons between means using the modified error mean square term (EMS) from the ANOVA 103

Structure-function analyses of the ATX1 metallochaperone

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