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Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures
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Cancer 42 , 3340 (2002)

Positive regulation of cell-cell and cell-substrate adhesion by protein kinase A

Journal Pathology 162, 803814

Real-time cell density assay Real-time cell density was measured using an xCELLigence detection system (ACEA Biosciences)
