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For example, colonic tryptophan hydroxylase 1 (Tph1) mRNA and protein were increased in humanized GF and conventionally raised mice

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doi: 10.1016/j.immuni.2013.02.012 96 VogelDYHeijnenPDBreurMde VriesHEToolATAmorSet al

If gentle rolling doesn't dissolve it within 5 minutes, let the vial sit at room temperature for 1530 minutes

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Bouslimani, A., Bec, N., Glueckmann, M., Hirtz, C