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However, long-term alcohol intake gradually transforms alcohol-induced autophagy into inhibition
Sanajou D, Ghorbani Haghjo A, Argani H, Aslani S (2018) AGE-RAGE axis blockade in diabetic nephropathy: current status and future directions
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Bowie, A
Lyse Sf9 cells expressing your protein of interest in ~20 ml of GST binding buffer (150 mM NaCl, 1 mM EDTA, 0.05% Triton-X-100, 1 mM DTT in PBS1X for a final concentration of NaCl of 250 mM, and protease inhibitor cocktail (Roche)
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