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Epithelial cells from Lgr5 DTR-EGFP/+ organoids were gated as EpCAMCD45 CD31 Ter119 7AAD , and Lgr5-EGFP fluorescence intensity was used to classify cells into GFPhigh and GFPneg populations, where the GFPneg gate was defined based on the background fluorescence intensity of WT control organoids lacking EGFP expression

The differential outcomes observed in Figure 3 suggest that the anxiolytic efficacy of the treatments hinges on their specific molecular configurations and the mechanisms by which they modulate neurobiological pathways

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[DOI] [PubMed] [Google Scholar] 64.Galani V

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Traditional antibiotic therapies, despite being the conventional approaches to eradicate these biofilm, often fail due to poor penetration into the biofilms deeper layers [30]