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18 mTORC1 integrates signals from nutrients and growth factors to regulate various anabolic processes, including protein synthesis, nucleotide production, lipid biosynthesis, and glycolysis, while inhibiting catabolic processes such as autophagy by phosphorylating ULK1/2 and sequestering lysosomal enzymes

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Importantly, these studies demonstrate the power of library-free DIA methods, which are necessary for the analysis of low cell numbers, given the limited sample availability for generating spectral libraries