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After isoflurane anesthesia mice were decapitated and brains were removed rapidly and placed in ice-cold artificial cerebrospinal fluid (ACSF) of the following composition (in mM): 130 NaCl, 24 NaHCO 3 , 3.5 KCl, 1.25 NaH 2 PO 4 , 0.5 CaCl 2 , 3 MgCl 2 and 10 glucose saturated with 95% O 2 /5% CO 2

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2021;15(4):1735-1744 | Article Number: 7262 Received : 20/08/2021 | Accepted : 29/09/2021 | Published : 15/10/2021 The elderly are more prone to mortality from COVID-19 infection, as they are susceptible to develop acute respiratory distress syndrome (ARDS)