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Cells were lysed for 15 minutes in ice-cold RIPA buffer consisting of 1% NP40, 0.1% SDS, 0.5% deoxycholate in PBS, supplemented with 1 mM PMSF, 1X cOmplete, EDTA-free Mini Protease Inhibitor Cocktail (Sigma Cat#11873580001) and 1X PhosSTOP (Sigma Cat#4906845001)

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doi: 10.1371/journal.pone.0009224 77 BewleyMAPrestonJAMohasinMMarriottHMBuddRCSwalesJet al

IV glutathione is administered cautiously in clients with reactive airway conditions due to a rare risk of bronchospasm
Weibin Zhang, [email protected]
