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The vector generated (pAAVS1-PC- GCLM ::Cherry) was confirmed by sequencing and co-transfected into HCT116 cells together with the AAVS1-TALEN-R/AAVS1-TALEN-L plasmids (Addgene #59026 and #59025, respectively)
3 The risk factors for methotrexate AEs include higher doses (3.5 g/m 2 ), rapid infusions, preexisting kidney impairment, older age (aged 50 years), and the concurrent use of medications that are nephrotoxic or that impair methotrexate clearance (eg, proton pump inhibitors, aminoglycosides, nonsteroidal anti-inflammatory drugs, penicillin and penicillin derivatives, amphotericin, probenecid)

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No study procedures were performed prior to obtaining informed consent

A recently published review that provides detailed descriptions of the dysregulation of extracellular glutamate, glutamate uptake changes in the acute stage of TBI and its role in chronic cognitive symptoms after TBI