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Detection of glutathione in cell culture medium A total 20 l of CAF-, LNCaP- or non-conditioned medium was mixed with 50 l acetone and incubated at 20 C overnight
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Additionally, the activity of GST was measured following the protocol outlined in Habig et al
It acts as an electron donor in reactions that neutralize free radicals, protecting cells and tissues from oxidative stress
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PLoS One 11 , e0165638 (2016)
