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The membrane was incubated with rabbit anti-GSS antigen affinity purified monoclonal antibody (M00928-2) at 1:500 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT

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