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Microglial NF-B activation under GSH-depleted conditions creates a pro-inflammatory loop where increased ROS promotes NF-B activation, which upregulates inflammatory cytokines and further stimulates oxidative stress (Liu et al., 2017

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Membranes were washed thoroughly with TBS-T and incubated with a secondary anti-mouse IgG horseradish-peroxidase-linked antibody (1:5,000 dilution) at 4 C for 2 h

[Google Scholar] Wei S., Van Griensven L

And combining methods creates its own set of variables

They contribute to the maintenance of mitochondrial function and cell viability by scavenging free radicals or safeguarding them through enzymatic reactions