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A 24 h time point was chosen based on a previous report of using this time period for transfecting or treating 3T3-L1 cells with NNMT anti-sense oligonucleotides or a small molecule NNMT product inhibitor (1-MNA), respectively, for phenotypic measures.[12] MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (ATCC

Best administered in the morning, when fat mobilization is naturally higher

Mild headaches, slight fatigue, or minor injection site irritation can occur as the body adjusts

They're excellent for body recomposition - losing fat while gaining muscle simultaneously

doi: 10.1016/j.drudis.2007.08.010