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ShHTLs of the different clades showed distinct responses to SL, including natural SLs: strigol, 5-deoxystrigol (5DS), 4-deoxyorobanchol and sorgolactone, and a racemic mixture of the synthetic SL analog GR24 ( rac -GR24) or KAR in cross-species complementation assays, in which Arabidopsis kai2-2 and htl-3 mutants were transformed with ShHTLs genes: ShHTL1 is not responsive to KAR or SL

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This process is regulated by the cytochrome P450 enzyme CYP7 A1, which catalyzes its rate-limiting step

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