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With smaller his-tagged proteins, several proteins(green with Hs) can bind to nickel ions (blue circles) on the same agarose bead

Levels of AST, ALT, creatinine, and oxidative stress markers including TBARS, SOD, CAT, GPx, TAS, TOS, GSH, and GSSG, along with pro-inflammatory cytokines IL-1, IL-6, IL-8, IL-10, IL-17, and TNF-, were measured using ELISA kits

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In this study, we performed a ChaC1-activity-directed pharmacological screening from FDA-approved drug library to identify GSH-sensitive agents, and found that ChaC1 overexpression mediated glutathione depletion largely enhanced auranofin (AUR)-induced cell death in hepatocellular carcinoma (HCC) cells

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Low levels of glutathione play a role in numerous disease conditions