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Effects of insufficient sleep on circadian rhythmicity and expression amplitude of the human blood transcriptome

Pipette 5 mL of the above solution into a 100 mL conical flask, add another 5 mL of 2% HPO solution to make a total volume of 10 mL, then add 1 mL of 5% potassium iodide and 2 drops of starch indicator

The bound beads are then washed three times with BC100 buffer (20 mM TrisCl, pH 7.9, 100 mM NaCl, 0.2 mM EDTA, 20% glycerol) containing 0.2% Triton X-100

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In literature NAC has been used in different doses and routes in different animal species (Supplementary Table 2)

Those with kidney disease should consult healthcare providers before using SS-31