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The DE algorithm adjusts the unknown coefficients of the proposed model by minimizing this objective function

Immunoblotting Protein extracts from adipose tissue mitochondria or cells were prepared using homogenization in radioimmunoprecipitation assay buffer containing protease inhibitors (Sigma-Aldrich)

These manifestations are associated with an increase of melanogenesis, hyperpigmented keratinocytes, senescent fibroblasts, vascularization and immune cells in particular mast cells as well as basement membrane disruption [32]

Futher changes are also made for the supplementary file supps.pdf, to make the main text more concise and coherent.

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Its potential in modulating ferroptosis in PCOS has attracted increasing research interest