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The cells were cultured in Dulbeccos modified Eagle medium supplemented with 10% fetal bovine serum (Gibco), 100 g/mL streptomycin, and 100 U/mL penicillin in a humidified incubator at 37C in the presence of 5% CO 2
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Its sharp flavor makes it a popular ingredient in dishes like fish curries, and its also used in place of lemon or tamarind to give recipes a pop of flavor
