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However, since MNs needed to accurately locate the sensor-tissue contact point, which would be very demanding on the operators skills, and miniaturized sensors might be interfered with by environmental noise, and multichannel systems needed to be supported by complex signal processing algorithms, data fusion was difficult, and the long-term electrochemical stability of the MN electrodes such as oxidation of alloys as well as the continued reliability of the sensors in organoid cultures still needed to be verified

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This effect correlated with increased apoptosis, evidenced by DNA fragmentation and higher cleaved caspase-3 and PARP levels