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The next day, fixed tissues were washed three times in Dulbeccos phosphate buffered saline (DPBS, GIBCO, #14190-094), and depending on the purpose, either embedded, frozen in optimal cutting temperature (OCT) compound (Sakura, #4583) and stored at 20 C, or stored at 4 C in PBS containing 0.2% gelatin (Sigma Aldrich, #G1393), 0.5% Triton X-100 (Sigma Aldrich, #X100) and 0.01% Thimerosal (Sigma Aldrich, #T8784) (PBS-GT)

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The studies specifically looking at soft tissue healing also spread the assessment of healing markers throughout the research period, involving data collection at various time points, for example, 1, 4, 7, 10 and 14 days throughout a 14-day study