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The following products were obtained from CHK Nutrition, Inc., Duluth, MN, USA

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Extracellular vesicles from patients with Acute Coronary Syndrome impact on ischemia-reperfusion injury

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Protein extraction and western blot analysis Cultured cells were lysed in radioimmunoprecipitation assay lysis buffer (Beyotime, Shanghai, China) supplemented with phenylmethylsulphonyl fluoride and protease inhibitor cocktail (Sigma-Aldrich, USA) on ice for 10 min and centrifuged at 12,000 g for 10 min at 4 C

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