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Importantly, these changes were observed without significant reductions in food consumption, pointing to enhanced energy metabolism rather than appetite suppression as the primary mechanism

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For the low dose group, a large number of collagen fibers were filled, the arrangement of collagen fibers was relatively loose and disordered compared with the PBS group

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In a duplicate, 1 ml of phosphate buffer was added to 2 ml of hemolyte, 1 ml of hydrogen peroxide (30 mm) solution was added after the spectrophotometry, and the absorbance was monitored at 750 nm for 3 min