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Analysis of the site 2 and site 3 leptinLepR interfaces also provides insight into the molecular basis of several human obesity-associated mutations in leptin, including D79Y , N82K , R84W , and S120C (Fig
Slides were visualized and photographed by an optical microscope (Nikon, Melville, NY, United States) at a magnification of 100 for histology, or by a confocal microscope (Leica, Bensheim, Germany) at a magnification of 20 for immunofluorescence
