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D., Hoang, V., Huang, J

Waterson, M.J

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35 , 961978 e910 (2023)

PCR was initiated by template denaturation at 95 C for 15 min, followed by 40 cycles of amplification (denaturation at 95 C for 10 s and primer annealing and extension at 60 C for 30 s)