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Phospho-peptide enrichment was performed using TiO 2 (Titansphere Phos-TiO Bulk 10 um, GL Science), ratio of beads to sample 10:1, mixed for 30 minutes at room temperature in 80% acetonitrile, 6% trifluoroacetic acid, 5 mM monopotassium phosphate, 20 mg/mL 2.5-dihydroxybenzoic acid

Extremes matter: patients under 50 kg often do well at the lower end, patients over 100 kg may sit at the upper end of standard ranges

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Liver Fat Accumulation: Helps detoxify the liver and prevent fat buildup

Results require patience, as hair growth cycles span months