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IL4 was also measured using the Mouse IL4 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) according to the manufacturer's protocol

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Researchers pull the vial, leave it at room temperature during preparation, then refrigerate again

FIGURE 7 Functional Annotation Study of PavGST Genes Gene ontology (GO) enrichment was evaluated in the biological process, subcellular localization, molecular function, and cellular components, which was beneficial for understanding protein function at the molecular level

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