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The protein concentrations were measured using the DC protein assay from Bio-Rad (Bio-Rad Laboratories, Hercules, CA, USA) and 40 g of cell protein was applied per lane to a 12% sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) and electrophoresed as described [20]

Those replicon-transfected cells that were superinfected with wt virus produced only background levels of Luc signal indicating a lack of trans-encapsidation by the wt capsid

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Cell 140(3), 313-326 (2010)

The great thing about the gym, to me, the that there is a social component to the guy

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