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The sample was again centrifuged at 1000 RCF for 10 min and the supernatant was used for protein quantification (Bradford protein assay, BioRad, Cat

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Rett syndrome (RS) is caused by mutations in the gene methyl-CpG-binding protein 2 (MECP2) Rett syndrome RS is characterized by language and communication problems, learning and coordination difficulties, and they display autistic-like behaviors [362, 363]

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