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SDS-PAGE, Immunoblotting, and mass spectrometry Equal cell numbers from liquid-grown cultures or equal amounts of CsCl fractions were solubilized in 2 Tris-Glycine SDS buffer (Life Technologies) by boiling for 15 min or heating at various temperatures

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Critically, the study documented no significant changes in oral glucose tolerance tests, no alterations in fasting insulin levels, and no changes in serum IGF-1 concentrations across all treatment groups
