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Generation and quantification of protein persulfides and polysulfides in cell lysates HEK 293 cell lysate (200 g) protein/sample in 50 mM HEPES buffer (pH 7.4) was treated with Na 2 S (040 M) or Na 2 S 2 (0600 M) for 7 min in sealed Eppendorf tubes with minimal head space at RT

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Consistent with a role as a pioneer factor, FOXH1 binds to DNA with extensive interactions over the minor and major grooves and shows higher affinity for its cognate sequence in nucleosomal DNA than in a linear DNA fragment

The same measure-and-adjust logic runs through his full supplement list

The house uses technology from marine and bridge construction as well as conventional building to create an elegant solution to flooding that is also attractive and complimentary to the setting