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Frank Welch, MD.
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Cell viability was evaluated by MTT assay, apoptosis by flow cytometry using annexin-V/propidium iodide staining, and protein expression of apoptosis-related markers (Bax, Bcl-2, and caspase-8) by western blot
Reconstituted 5-Amino-1MQ should be kept at 28C, protected from light, and used within 30 days

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