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This dynamic range is higher than recently developed biosensors such as the FiLa lactate sensor (1,500%-2,700%) (Li et al., 2023), iATPSnFR2 ATP sensor (1200%)(Marvin et al., 2024), or the redox biosensors roGFP2 (600-1200%)(Pedre, 2024), HyPer7 (500%)(Pak et al., 2020), SoNar (1500%)(Zhao et al., 2015) or iNap (900%) (Tao et al., 2017)

freudenreichii are autonomous anaerobic strains that produce cobalamin through microbial biosynthesis

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Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast

In order to change the GSH in the lysis buffer for other redox-active compounds, proteins were desalted with a MiniTrap column