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One of the earliest events associated with APAP intoxication and excessive NAPQI generation is depletion of GSH stores, both the cytosolic and mitochondrial pools (45)

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For the pellet fractions (P1 and P2), we added 500 and 125 L of reagent B (75% dichloromethane (Fisher Scientific, AA22917K2 HPLC-grade) and 25% methanol), respectively

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